Benjamin Herzog

I hereby certify that Mr. Benjamin Herzog, a student of biology at the Humboldt University Berlin, in July 2011, began with the work on his master thesis with the On "investigation to protein-protein interactions of plant Sucrose transporter ", under my care at the Department of Biology AG Plant Physiology by Prof. B. Grimm.
As part of his master thesis came in molecular biology and standard physiological techniques used. This included the DNA isolation, plasmid isolation, restriction digestion, PCR, Gel electrophoresis, RNA isolation, real-time qPCR, yeast transformation and complementation, plasmid isolation from E. coli, Agrobacterium, Yeasts, cloning, purification of DNA from the gel Menten question, Working with sterile tissue culture propagation and amplification transgenic plants, review of successful transformation, Determine the subcellular localization of GFP fusion proteins using confocal laser scanning microscopy (CLSM).
It gained experience with the yeast two hybrid split ubiquitin A system for detection of protein interactions of plant Membrane proteins and confirmed this using bimolecular fluorescence Complementation (BiFC) in planta. He learned both the transient Agrobacterium-mediated transformation of tobacco leaves by Infiltration, and the stable transformation of Solanum tuberosum (Karoffel) plants using an RNA interference (RNAi) Construct, which he independently within the scope of his master's thesis produced.
It was completed by him in the internships also his knowledge deepen in protein purification and expression.
The progress of his experiments he placed at regular Intervals of the Working Group employees seminar in English before.
I know Mr. Herzog because of his participation in several courses and already noticed in the course of his studies, seminars and drew always valuable lectures by well-developed and discussion from the respective seminars. I recommend him therefore fully as an assistant and PhD student.

lab experience:

DNA-/RNA-/Plasmid-/Protoplastenisolierung, restriction digestion, PCR, Gel electrophoresis, reverse transcription, cDNA synthesis, real-time qPCR, E. coli / A. tumefaciens / plant / S.cerevisae / Cyanobacteria transform Cloning,-cultivating, working with sterile tissue culture Protein expression, purification, activity measurements, Western blot, HPLC ÄKTA, in vivo imaging, Fluorescence measurement, affinity chromatography, Laser scanning microscopy, cloning and common office programs

legal examens:

Erstes Staatsexamen VB
Zweites Staatsexamen B

intellectual property law
civil law